Cell Motility: Measurements of Ciliary Beat Frequency.
Primary Airway Ciliated Epithelial Cell Services
BioTechPlex provides to industry, academia and government, the evaluation of targeted agents on ciliary beat frequency using our Cilscreen™ and Ciliascope™ technologies.
The effects of the selected agents are tested using primary cultures of canine, ovine or porcine ciliated airway epithelial cells, CiliScreen™ . These ciliated airway epithelial cells sustain robust, synchronous beating for weeks. The cell morphology resembles those found in in vivo conditions as show below.
Examples of scanning electron micrographs of one month old primary canine tracheal epithelial cell cultures
Measurements of Ciliary beat frequency, CBF, are made with Ciliascope ™ . Ciliascope ™ is an epi-illumination inverted microscope specifically designed for the real-time measurement of ciliary beat frequency (CBF). Ciliascope ™ uses dynamic laser light scattering and nonstationary signal analysis to measure, display, and record CBF as the experiment is being performed. Ciliascope ™ measures CBF in every 1 to 2 seconds. Thus responses of ciliary beat to the test agent can be measured in very short (seconds) and long (hours) times frames according the agent's mode of action. The followings are typical stimulatory an inhbitory responses of Cilscreen measured by the Cilascope.
Applications of our Airway Epithelial Cell Products and Services
• Airway disease research
• Drug discovery and development
• Cost effective alternative to pre-clinical whole-animal screening
• Toxicology
Ceraltics scientific staff will help you define your needs, plan your studies, perform the experiments, provide the raw and analyzed data and assist in its interpretation.
For more information, please contact: var contact = "Sales" var email = "sales" var emailHost = "biotechplex.com" // document.write(" " + contact + " ") Sales or call 760 744 1000 or fax 760 744 0033 .
Acknowledgements: The research and development of the cultured ciliated cells are supported by R43 & R44 HL 67595 from the Heart Lung and Blood Institute of the National Institutes of Health, USA . The research and development of the Ciliascope ™ are supported by R43 & R44 HL 59126 from the Heart Lung and Blood Institute of the National Institutes of Health, USA
BioTechPlex enables pharmaceutical and biotechnology companies to gain a competitive advantage by providing access to novel technologies and cell lines for ionic mechanism-dependent drug discovery, validation and safety pharmacology. Although not limited to respiratory and cardiovascular diseases, BioTechPlex personnel have advanced expertise in this arena.
- Integration of cell, tissue and animal physiology, pharmacology, toxicology and drug safety
- Early identification of cyotoxic agents
- Consolidation of 2 ° & 3 ° screens, with increased speed and throughput.
- Reduction of out-of-pocket costs and drug development time.
CardioDataServe for HRV analysis of pre-recorded ECG's
Send your ECG data to CardioDataServe for complete trouble free analysis of Heart Rate Variability.
For more information, please contact:
760 744 1000 or fax 760 744 0033 .
